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Gibco™ MAPKAPK3 Recombinant Human Protein, Inactive
MAPKAPK3 Recombinant Human Protein, Inactive
Marke: Gibco™ PV3300
Additional Details : Gewicht : 3.36000kg
Beschreibung
MAPKAPK3 functions as an integrative element of signaling in both mitogen and stress responses.Spezifikation
| NP_004626 | |
| Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 47.3kDa | |
| 100μg | |
| For research use only. Not for use in diagnostic procedures | |
| MAPKAPK3 | |
| Both the active and inactive enzymes were diluted in enzyme dilution buffer and the reaction was set up in two stages. In stage one, MAPKAPK3, inactive (3.33μg/mL final concentration) was incubated with MAPK14 (PV3304) (0.83μg/mL final concentration) or with dilution buffer for basal MAPKAPK3, inactive activity in 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% NP-40, 500μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix was transferred to 25μL of reaction mix containing 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% NP-40, 500μM ATP, 33μM MAPKAPK3 kinase substrate peptide and trace [32P]-γ-ATP and incubated for 10 minutes at 30°C | |
| MAPKAPK3 Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
| 0.98mg/mL | |
| 7867 | |
| MAPKAPK3 Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| MAPKAPK3 Recombinant Human Protein | |
| MAPKAPK | |
| ∽2% activity as compared to the active form of the kinase | |
| His Tag | |
| Kinase and inhibitors | |
| 95% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.