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![MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein 10μg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-650.jpg)
MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein
MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein
Marke: Invitrogen PV3293
Additional Details : Gewicht : 3.36000kg
Beschreibung
MAP2K6 (MKK6) is a serine⁄threonine kinase that functions as an activator of p38 in the MAPK pathway. The MAP2K6 S207E T211E mutant is constitutively active.Spezifikation
| NP_002749, S207E, T211E | |
| Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 41.7kDa | |
| 10μg | |
| For research use only. Not for use in diagnostic procedures | |
| MAP2K6 (MKK6) S207E T211E | |
| MAP2K6 (MKK6) S207E T211E was assayed in a two stage coupled reaction involving phosphorylation of MAPK14 inactive (PV3305) by MAP2K6 (MKK6) S207E T211E followed by phosphorylation of MBP substrate by activated MAPK14. The enzymes were diluted in enzyme dilution buffer before adding to the reaction. In stage one, MAP2K6 (MKK6) S207E T211E at 0.83μg/mL final concentration, is incubated with MAPK14 inactive at a final concentration of 35μg/mL in 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% Triton™ X-100, and 200μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix was transferred to 25μL of a reaction mixture containing 50mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 2mM DTT, 0.01% Triton™ X-100, 200μM ATP, 0.667μg/μL MBP substrate and trace [ 32P]-γ-ATP and incubated for 10 minutes at 30°C | |
| Full length (S207E, T211E) | |
| 3810nmoles of phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mg of total protein Activity determined at a final protein concentration of 0.139μg/mL | |
| His Tag | |
| Kinase and inhibitors | |
| 90% by SDS-PAGE |
| 0.3mg/mL | |
| 5608 | |
| MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein | |
| STE7 | |
| MAP2K6 (MKK6) [S207E] [T211E] Recombinant Human Protein | |
| Human | |
| E. coli | |
| Serine/threonine kinase |
For Research Use Only. Not for use in diagnostic procedures.