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Thermo Scientific™ MabCaptureC Affinity Matrix
Alle ansehen Thermo Scientific ProdukteBeschreibung
MabCaptureC Affinity Matrix is an agarose-based, high-binding-capacity Protein A chromatography resin with a rProtein A ligand produced in yeast. The engineered rProtein A binds at the CH2-CH3 interface of the Fc-region of IgGs and shows cross binding to the VH3 domain of IgGs.
MabCaptureC™ Affinity Matrix is suitable for the purification of monoclonal antibodies.The rProtein A ligand is immobilized on a highly cross-linked and uniform-sized agarose-based bead with an average particle size of 75 μm. The uniform particle size delivers superior performance characteristics compared to traditional Protein A resins.
Features of the MabCaptureC™ Affinity Matrix include:
- High dynamic binding capacity for mAbs of >50 g/L at 4.8 min residence time
- Purification of mAbs and a subpopulation of scFv's and Fab fragments containing VH3 binding domains
- Clean and sanitize with standard sodium hydroxide agents for chromatography products
- Excellent scalability
- Non-animal derived
Main characteristics
- Matrix: agarose-based, epoxide activated
- Average particle size: 75 ± 10 μm
- Ligand: recombinant C domain of Protein A
- Ligand coupling method: epoxide coupling
- Binding capacity: >50 g IgG/L resin at 4.8 min residence time and 10% breakthrough
- Elution conditions: 0.1 M glycine pH 3.0, or 20–100 mM sodium citrate or acetate, pH 3.0
- Cleaning conditions: 0.2 M sodium hydroxide for 15 minutes every cycle
- Operating pressure: ≤3 bar
- Pressure flow: >500 cm/h at 3 bar in a 2.6 x 20 cm column
- Formulation buffer: 20% (v/v) ethanol
For process development and resin screening, MabCaptureC™ is also available in the following formats:
- MabCaptureC™ MiniChrom Column, 1 mL (Cat. No. 5943662001)
- MabCaptureC™ MiniChrom Column for process development, 5 mL (5943662005)
- MabCaptureC™ RoboColumn, 200 μL (5943662200)
- MabCaptureC™ RoboColumn, 600 μL (5943662600)
Spezifikation
Spezifikation
| Produkttyp | Affinity Matrix |
| Bindungsvermögen | >50 g IgG/L |
| Ligand | Engineered rProtein A |
| Matrix | Agarose-based, epoxide-activated |
| Partikelgröße | 75 ±10 μm |
| Ziel | CH2-CH3 interface Fc-region |
| Zusammensetzung | Suspension in 20% ethanol |
| Zur Verwendung mit (Anwendung) | Monoclonal antibody purification |
| Menge | 250 mL |
| Form | Agarose Bead |
Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.
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