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Ly-49G2 Rat anti-Mouse, PerCP-eFluor™ 710, Clone: eBio4D11 (4D11), eBioscience™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 46-5781-80

Additional Details : Gewicht : 0.25000kg

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Artikelnummer. 15321350

  • 136.00 CHF / 25 Mikrogramm


Verfügbar ab
20-04-2021
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Beschreibung

Beschreibung

Description: The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor. Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activatingreceptors. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur. Applications Reported: This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
Spezifikation

Spezifikation

Ly-49G2
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60654
Killer cell lectin-like receptor 7, Lymphocyte antigen 49g, Ly-49g, T-cell surface glycoprotein Ly-49G, Klra7, Ly-49g, Ly49-g, Ly49g, Ly49g4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
eBio4D11 (4D11)
PerCP-eFluor™ 710
Klra7
Liquid
Klra7
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16638
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.