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LS1034 (human cecal carcinoma) Whole cell lysates, Denatured; Abnova

Artikelnummer. 16029625
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Menge:
200 μg
Unit Size:
200 Mikrogramm
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16029625 200 μg 200 Mikrogramm
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Product Code. 16029625 Supplier Abnova Supplier No. L049V1.200ug

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Description

Western Blotting

Specifications

Zur Verwendung mit (Anwendung) Western Blot
Tissue Cecum
Beschreibung Whole cell lysate (denatured)
Lysepuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Vorbereitungsmethode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Qualitätskontrollen 12.5% SDS-PAGE Stained with Coomassie Blue.
Menge 200 μg
Lagerungspuffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Wirtsspezies Human
Konzentration 3 mg/mL
Inhalt und Lagerung Lagerung bei -80 °C. Aliquot, um wiederholtes Einfrieren und Autauen zu vermeiden.
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