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LNCaP clone FGC (human prostate carcinoma) Whole cell lysate, Denatured; Abnova
Marke: Abnova L004V1.200ug
Additional Details : Gewicht : 0.02000kg
Beschreibung
- Whole cell lysate (denatured)
- Tissue: prostate
- Host: Human
- Preparation: homogenization in ice-cold modified RIPA Lysis
- Lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 1mM PMSF
- Storage buffer: in ready-to-use 1X sample buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% bromophenol blue)
Western blotting
Spezifikation
| Western Blot | |
| Whole cell lysate (denatured) | |
| Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. | |
| 200 μg | |
| Human | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Prostate | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). | |
| 3 mg/mL |