missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Reagenzien für die Proteinanalyse
  4. Lysate
  5. Zelllinienlysate
  6. 16069545

Jurkat, clone E6-1 (human acute T cell leukemia) Whole cell lysate, Non-denatured; Abnova

Artikelnummer. 16069545
Click to view available options
Menge:
200 μg
Packungsgröße:
200 Mikrogramm
Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen

Artikelnummer. 16069545

Marke: Abnova L017V2.200ug

om dit product te kopen Registreer vandaag om een webaccount aan te maken

Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen

Description

Non-denatured whole cell lysate of Jurkat, clone E6-1 (human acute T cell leukemia)

  • Tissue: T Lymphocyte
  • Host: Human
  • Lysis Buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

Zur Verwendung mit (Anwendung) Immunoprecipitation, Western Blot
Tissue T Lymphocyte
Beschreibung Whole cell lysate (non-denatured)
Lysepuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Vorbereitungsmethode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Qualitätskontrollen 12.5% SDS-PAGE Stained with Coomassie Blue.
Menge 200 μg
Lagerungspuffer In modified RIPA Lysis Buffer.
Wirtsspezies Human
Inhalt und Lagerung Lagerung bei -80 °C. Aliquot, um wiederholtes Einfrieren und Autauen zu vermeiden.
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.