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IRF7 Rat anti-Human, PE, Clone: RDP4ND4, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 12-5375-42

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15963174

  • 387.00 CHF / 100 Stück


Voraussichtliches Versanddatum: 30-09-2021
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Beschreibung

Beschreibung

Description: The monoclonal antibody RDP4ND4 recognizes human interferon regulatory factor 7 (IRF7), a 54 kDa member of the interferon regulatory transcription factor family. It is essential for the antiviral innate immune response and the activation of type I IFN genes. IRF7 is constitutively expressed in the cytoplasm of plasmacytoid dendritic cells, B cells and monocytes, but it is strongly induced in response to virus infection and interferon stimulation. IRF7 can activate IFN-beta and IFN-alpha genes via the virus-activated, MyD88-independent pathway, or the TLR-activated, MyD88-dependent pathway. Virus exposure induces IRF7, through toll-like receptors TLR7, TLR8, and TLR9, to promote IFN gene expression and disrupt infection. IRF7 exists in an inactive form until phosphorylated and its phosphorylation leads to dimerization and nuclear translocation of IRF7 where it aids in activating target genes. The IRF7 gene is upregulated by interferon stimulation through the Jak-STAT pathway in a positive feedback loop. Applications Reported: This RDP4ND4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RDP4ND4 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523) and protocol.

Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
Spezifikation

Spezifikation

IRF7
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q92985
IRF7
IgG2a, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3665
Flow Cytometry
RDP4ND4
PE
IRF7
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate