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  7. Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Codice prodotto. 10704274
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N. di reazioni:
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50 Reactions
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250 reazioni
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Codice prodotto. 10704274 Fornitore Invitrogen™ N. del fornitore 11754250
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Descrizione

Includes

5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage

• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL

Store at –5 to –30°C.
Metodo di rilevamento qPCR
Formato Kit
GC-Rich PCR Performance High
Reaction Speed 60 min.
Tecnica Reverse Transcription
Optimal Reaction Temperature 42°C
Quantità 250 reactions
Reverse Transcriptase SuperScript III
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Da utilizzare con (applicazione) Real Time PCR (qPCR)
Tipo di prodotto finale First-Strand cDNA
N. di reazioni 250 Reactions
Reaction Format Master Mix
Tipo di reagente Reverse Transcription
Dimensioni (prodotto finale) Up to 10 kb
Starting Material RNA
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For Research Use Only. Not for use in diagnostic procedures.

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