Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase

• Sensitive detection down to 0.01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size – compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript III Reverse Transcriptase
SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.

Note: This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.