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Invitrogen™ Taq DNA Polymerase, recombinant

Codice prodotto. 10174762
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10174762 100 Reactions Pezzo
10033382 500 Reactions 500 unità
11508626 1500 Reactions 1000 unità
10063262 5000 Reactions Pezzo
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Codice prodotto. 10174762 Fornitore Invitrogen™ N. del fornitore 10342053
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Includes

Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 5 U/μL
Content And Storage • Taq DNA Polymerase (5 U/μL), 20 μL
• 10X PCR buffer, 1.5 mL
• 50 mM MgCl2, 1 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.
Formato Stand-alone enzyme
GC-Rich PCR Performance Low
PCR Method qPCR, Standard PCR
Polimerasi Taq DNA Polymerase
Reaction Speed Standard
Attività esonucleasi 5' - 3'
Product Type DNA Polymerase
Quantità 100 Units
Shipping Condition Dry Ice
Da utilizzare con (applicazione) Standard PCR
Fedeltà (rispetto a taq) 1X
Hot Start No
N. di reazioni 100 Reactions
Sporgenza 3'-A
Reaction Format Separate Components
Dimensioni (prodotto finale) 5 kb or less
Starting Material DNA
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For Research Use Only. Not for use in diagnostic procedures.

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