Gibco™ High Five™ Cells in Express Five™ Medium

High Five Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichopulsia ni cell line (cabbage looper ovary). High Five Insect Cells are adapted to serum-free culture in Express FiveSFM for maximum cell growth and recombinant gene expression.

• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems High levels of protein expression in High Five cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System, or the InsectDirect™ Expression System. Up to ten-fold increase in secreted protein expression compared to Sf9 cells High Five cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells. Good growth in adherent or suspension culture High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express FiveSFM are available in the product manual. High Five cells can be adapted to suspension culture. Quality and performance testing Each lot of High Five cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Bioproduction, Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Insect Expression, Insect Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Vaccine Production