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Invitrogen™ β-Gal Assay Kit

Codice prodotto. 10093072
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Quantità:
80 mL
Dimensione della confezione:
5mL
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Codice prodotto. Quantità Dimensione della confezione
10093072 80 mL 5mL
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Codice prodotto. 10093072 Fornitore Invitrogen™ N. del fornitore K145501
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Verfügbar ab: 03-12-2026
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Includes

10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.

Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene

  • lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
  • Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
  • β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
  • Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
  • 10.7 gene expression
  • Colorimetric detection

Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays

TRUSTED_SUSTAINABILITY

Specifica

Content And Storage The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Saggio β-Gal (lacZ) Assay
Metodo di rilevamento Colorimetric
Product Type Beta-Gal Assay Kit
Substrato ONPG
Quantità 80 mL
Bersaglio Beta-Galactosidase

For Research Use Only. Not for use in diagnostic procedures.

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