Gibco™ FreeStyle™ 293-F Cells

Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle MAX 293 Expression System or FreeStyle 293 Expression System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle 293 Expression Medium. The FreeStyle 293-F cell line is supplied in a vial containing 1mL of cells at 1 x 10⁷ viable cells/mL in 90% FreeStyle 293 Expression Medium and 10% DMSO

Characteristics of FreeStyle 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were recloned by limiting dilution
• The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle 293 Expression Medium
• Demonstrate high transfection efficiencies with FreeStyle MAX reagent
• Suspension cultures may be transfected in FreeStyle 293 Expression Medium without the need to change media
• Permits transfection of cells at large volumes
• Voltage limit: 0.5-2.5kV

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection