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Invitrogen™ Insulin Human ELISA-Kit

Artikelnummer. 10039272
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Menge:
96 Tests
Unit Size:
96 Tests
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Product Code. Menge unitSize
10039272 96 Tests 96 Tests
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Product Code. 10039272 Supplier Invitrogen™ Supplier No. KAQ1251
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Description

ELISA

The Human Insulin ELISA Kit quantifies human insulin in serum. The assay will recognize both the natural and recombinant insulin. Principle of the method The Human insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
TRUSTED_SUSTAINABILITY

Specifications

Zugriffsnummer Gehäuse P01308
Assay-Bereich 5.1-250 μlU/ml
Assay-Empfindlichkeit: 0,17 μIU/ml
Inhalt und Lagerung Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugat Biotin
Immunassay-Kit-Format Sandwich ELISA Kit
Produkttyp ELISA-Kit
Proteinfamilie Hormones & Receptors
Probentyp Serum
Zielspezies Human
Zur Verwendung mit (Anwendung) Kolorimetrischer Nachweis
Zur Verwendung mit (Geräte) Kolorimetrisches Mikrotiterplatten-Lesegerät
Gen-Alias AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulinAA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin; IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10
Gen-ID (Entrez) 3630
Gensymbol IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Inkubationszeit 0.75 h
Interassay-VK 8.5 %
Intraassay-VK 5.4 %
Kitinhalt Vorbeschichtete 96-Well-Platte, Standard, Inkubationspuffer, Biotinylierter Nachweisantikörper, Biotinylierter Nachweisantikörper, Waschpuffer, Stopplösung, Waschpuffer, Chromogen, Stopplösung, Kontrollen
Marker oder Farbstoff HRP
Markergen Insulin
Menge 96 Tests
Kennzeichnung RUO
Probenvolumen Serum (50 μl)
Versandbedingung Wet Ice
Lagerungsbedingungen 2 bis 8 °C
Ziel Human
Prüfzeit 1 h 20 min
Gesamte Assay-Zeit 45 Min.
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Safety and Handling

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Product Identifier
  • Insulin Human ELISA Kit
 Signal Word
  • Gefahr
 Hazard Category
  • Schwere Augenschädigung/Augenreizung Kategorie 1
  • Hautreizende/-ätzende Wirkung Kategorie 1B
 Hazard Statement
  • H314-Verursacht schwere Verätzungen der Haut und schwere Augenschäden.
  • H318-Verursacht schwere Augenschäden.
 Precautionary Statement
  • P260-Staub/Rauch/Gas/Nebel/Dampf/Aerosol nicht einatmen.
  • P264-Nach Gebrauch gründlich waschen.
  • P280-Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.
  • P301+P330+P331-BEI VERSCHLUCKEN: Mund ausspülen. KEIN Erbrechen herbeiführen.
  • P303+P361+P353-BEI BERÜHRUNG MIT DER HAUT (oder dem Haar): Alle kontaminierten Kleidungsstücke sofort ausziehen. Haut mit Wasser abwaschen/duschen.
  • P304+P340-BEI EINATMEN: Die Person an die frische Luft bringen und für ungehinderte Atmung sorgen.
  • P305+P351+P338-BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
  • P310-Sofort GIFTINFORMATIONSZENTRUM/Arzt/anrufen.
  • P363-Kontaminierte Kleidung vor erneutem Tragen waschen.
  • P501b-Inhalt/Behälter gemäß den örtlichen/regionalen/nationalen/internationalen Vorschriften zuführen.
 Supplemental information
  • MIXTURE LIST-Contain: Hydrogen chloride
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