Invitrogen™ Image-iT™ Low Density Lipoprotein Uptake Kit, Bodipy FL

The kit was designed to specifically detect binding and uptake of LDL through the LDL receptor internalization pathway, allowing maximum control and flexibility in the experimental design. It can be used with fluorescence microscopy, flow cytometry, cell sorting, high-throughput analysis, and high content analysis.

• Optimized LDL uptake kit with two built-in controls
• Bodipy FL is detectable with GFP/FITC filter set (absorption peak: ∼515 nm, emission peak: ∼520 nm)
• Bodipy FL is fixable and multiplexable with orange, red, deep red, violet, and blue emission dyes, such as RFP, mCherry, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

Two controls are included in the kit: unlabeled LDL as an optional pretreatment to occlude cell surface receptors in advance of probing with the labeled construct, and heparin which will chelate the LDL in the solution phase outside of the cells, preventing binding and uptake. When cells are labeled in the absence of these pretreatment controls, a fluorescent signal indicates specific LDL receptor-mediated endocytosis.