IL-4 Rat anti-Human, Biotin, Clone: MP4-25D2, eBioscience™
Rat Monoclonal Antibody
Marke: Affymetrix eBioscience 13-7048-81
Additional Details : Gewicht : 0.25000kg
Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry. Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombit human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402|
|4° C, do not freeze|
For Research Use Only.