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  5. Invitrogen™ Mouse IL-3 ELISA Kit

Invitrogen™ Mouse IL-3 ELISA Kit

Artikelnummer. 17165749
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Menge:
10 x 96 Tests
5 x 96 Tests
96 Tests
Packungsgröße:
1 Set
1 Stück
10 Stück
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Artikelnummer. 17165749 Lieferant Invitrogen™ Lieferanten-Nr. EMIL3X5
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Beschreibung

ELISA

The Mouse Interleukin-3 (Ms IL-3) ELISA quantitates Ms IL-3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-3. Principle of the method The Mouse IL-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
TRUSTED_SUSTAINABILITY

Spezifikation

Zugriffsnummer P01586
Assay-Bereich 0,16 pg/mL bis 40 pg/mL
Assay-Empfindlichkeit: 0,2 pg/mL
Kalibrierbereich 0,2 pg/mL bis 40 pg/mL
Assay Festphasen-Sandwich-ELISA
Konjugat Biotin
Immunassay-Kit-Format Sandwich ELISA Kit
Produkttyp ELISA-Kit
Proteinfamilie Zytokine und Rezeptoren
Probentyp Plasma, Serum, Überstand
Spezifität Dieses ELISA-Kit zeigt keine Kreuzreaktivität mit den folgenden getesteten Zytokinen: Maus CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fraktalkin, GCSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, KC, Leptin R, LEPTIN (OB), LIX, L-Selektin, Lymphotactin, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selektin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF.
Ausreichend für 5 x 96 Assays
Zielspezies Maus
Zur Verwendung mit (Anwendung) ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse
Zur Verwendung mit (Geräte) Kolorimetrisches Mikrotiterplatten-Lesegerät
Gen-Alias BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSFBP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF; BPA; Csfmu; HCGF; Il-3; MCGF; PSF
Gen-ID (Entrez) 16187
Gensymbol BPA,Csfmu,HCGF,Il-3,MCGF,PSF
Interassay-VK <12%
Intraassay-VK <10%
Kitinhalt Chromogen, Standard, Assay-Verdünnungsmittel-Konzentrat, SAV-HRP, Dichtungsfolien für Mikrotiterplatten, Vorbeschichtete 96-Well-Platten, Stopplösung, Waschpuffer, Biotinylierter Nachweisantikörper
Marker oder Farbstoff HRP
Menge 5 x 96 Tests
Kennzeichnung RUO
Forschungsgebiet Immunologie
Probenvolumen Plasma, 50 μl; Serum, 50 μl; Überstand, 100 μl
Versandbedingung Wet or Dry Ice
Lagerungsbedingungen 2 bis 8 °C
Synonym RP23-309E16.5, BPA, Csfmu, HCGF, Il-3, MCGF, PSF
Ziel Maus
Prüfzeit 1 h 20 min
Gesamte Assay-Zeit 4 h 45 min
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