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Invitrogen™ IL-3A Human ELISA-Kit

Artikelnummer. 10426673
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Menge:
96 Tests
Packungsgröße:
1 Stück
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10426673 96 Tests 1 Stück
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Artikelnummer. 10426673 Lieferant Invitrogen™ Lieferanten-Nr. KHC0031
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Verfügbar ab: 27-08-2026
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Beschreibung

ELISA

The Human Interleukin 3 (Hu IL-3) ELISA quantitates Hu IL-3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-3. Principle of the method The Human IL-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
TRUSTED_SUSTAINABILITY

Spezifikation

Zugriffsnummer Gehäuse P08700
Assay-Bereich 7.8 bis 500 pg/ml
Assay-Empfindlichkeit: <1 pg/ml
Inhalt und Lagerung Vorbeschichtete Platte(n), Standard oder Kalibrator, Nachweisantikörper, HRP-Konjugat, Verdünnungsmittel, Waschpuffer, Substrat(e) und Stopplösung. Kit bei 2 bis 8 °C lagern. Detaillierte Informationen zu Inhalt und Lagerbedingungen für maximale Stabilität finden Sie im Produkthandbuch.
Konjugat Biotin
Immunassay-Kit-Format Sandwich ELISA Kit
Produkttyp ELISA-Kit
Proteinfamilie Cytokines & Receptors
Probentyp Plasma, Serum, Überstand
Zielspezies Human
Zur Verwendung mit (Anwendung) Kolorimetrischer Nachweis
Zur Verwendung mit (Geräte) Kolorimetrisches Mikrotiterplatten-Lesegerät
Gen-Alias BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSFBP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF; IL-3; MCGF; MULTI-CSF
Gen-ID (Entrez) 3562
Gensymbol Il-3, MCGF, MULTI-CSF
Inkubationszeit 4 h
Interassay-VK 7.3 %
Intraassay-VK 5.5 %
Kitinhalt Vorbeschichtete 96-Well-Platte, Standard, Biotinylierter Nachweisantikörper, Biotinylierter Nachweisantikörper, Waschpuffer, Stopplösung, Waschpuffer, Chromogen, Stopplösung, Dichtungsfolien für Mikrotiterplatten
Marker oder Farbstoff HRP
Markergen Gehäuse IL-3
Menge 96 Tests
Kennzeichnung RUO
Probenvolumen Plasma, 100 μl; Serum, 100 μl; Überstand, 100 μl
Versandbedingung Wet Ice
Lagerungsbedingungen 2 bis 8 °C
Ziel Human
Prüfzeit 1 h 20 min
Gesamte Assay-Zeit 4 h
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