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  5. Invitrogen™ Mouse IGF-1 ELISA Kit

Invitrogen™ Mouse IGF-1 ELISA Kit

Codice prodotto. 18597774
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Quantità:
10 x 96 Tests
5 x 96 Tests
Dimensione della confezione:
Pezzo
pezzi 10
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Codice prodotto. Quantità Dimensione della confezione
18597774 10 x 96 Tests pezzi 10
17135739 5 x 96 Tests Pezzo
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Codice prodotto. 18597774 Fornitore Invitrogen™ N. del fornitore EMIGF1X10
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    5990.00 CHF
    5810.30 CHF / 10 Stück
    Sparen 179.70 CHF
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Verfügbar ab: 16-12-2026
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Descrizione

ELISA

The Mouse Insulin-like Growth Factor 1 (Ms IGF-1) ELISA quantitates Ms IGF-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGF-1. Principle of the method The Mouse IGF-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
TRUSTED_SUSTAINABILITY

Specifica

Numero di accesso P05017
Intervallo saggio 4-2000 pg/mL
Assay Sensitivity 4 pg/mL
Intervallo di calibrazione 4 pg/mL to 2000 pg/mL
Saggio Solid-phase sandwich ELISA
Coniugato HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Famiglia di proteine Growth Factors & Receptors
Tipo di campione Plasma, Serum, Supernatant
Specificità This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sufficiente per 96 Assays
Target Species Mouse
Da utilizzare con (applicazione) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Da utilizzare con (apparecchiatura) Absorbance Microplate Reader
Alias gene C730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-CC730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-C; C730016P09Rik; Igf-1; Igf-I
ID gene (immissione) 16000
Simbolo del gene IGF1
Interassay CV <12%
Intraassay CV <10%
Contenuto kit Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases IGF-I, Somatomedin
Quantità 10 x 96 Tests
Status giuridico RUO
Disciplina di ricerca Development
Volume del campione Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Requisiti di stoccaggio Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Sinonimo C730016P09Rik, Igf-1, Igf-I
Bersaglio IGF1
Durata del test 1 hr 20 min
Tempo totale saggio 4 hr 45 min
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