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Invitrogen™ Human IFN alpha Matched Antibody Pair

Artikelnummer. 15501707
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Menge:
10 x 96 Tests
Packungsgröße:
10 Stück
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15501707 10 x 96 Tests 10 Stück
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Description

ELISA

The Human IFN alpha Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IFN alpha. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
TRUSTED_SUSTAINABILITY

Specifications

Zugriffsnummer P01562, P01563
Antigen IFN alpha
Assay-Bereich 7.8-500 pg/mL
Anwendungen ELISA
Assay-Empfindlichkeit: 7.8 pg/mL
Klassifikation Monoclonal
Konjugat HRP
Gen-Alias Alpha interferon, IFN leukocytic, LeIF D, alpha-2a interferon; Gm12597; ifa; Ifa1; Ifa2; IFL; IFN; IFN alpha; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN1@; IFNA; IFNA@; IFNA1; IFNA13; Ifna15; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFNA3; IFNA6; Ifnaa; IFNAalphaIFN-alpha-1/13; IFNalpha; IFN-alpha; IFN-alpha 1b; IFN-alpha1; IFN-alpha-1; IFN-alpha-1/13; IFN-alpha-2; IFN-alpha-6; IFN-alphaA; IFN-alphaD; IFN-gamma; IFNα; INFA2; interferon; interferon alpha; interferon alpha 1; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha 1b; interferon alpha 2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); interferon alpha protein; interferon alpha-1; Interferon alpha1 (IFNA1); interferon alpha-1 precursor; interferon alpha-1/13; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-2; Interferon alpha-A; Interferon alpha-D; Interferon alpha-D (IFN alphaD); interferon type A1/A2; interferon type A3; interferon, alpha 1; interferon, alpha 2; interferon-alpha; interferon-alpha 1; interferon-alpha1; interferon-alpha-6; leIF A; LeIF D; MGC138207; MGC138505; MGC138507; MuIFN-alpha-A; novel interferon alpha (Ifna) family member; OTTMUSG00000007655alpha-2a interferon; Gm12597; ifa; Ifa1; Ifa2; IFL; IFN; IFN alpha; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN1@; IFNA; IFNA@; IFNA1; IFNA13; Ifna15; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFNA3; IFNA6; Ifnaa; IFNAalphaIFN-alpha-1/13; IFNalpha; IFN-alpha; IFN-alpha 1b; IFN-alpha1; IFN-alpha-1; IFN-alpha-1/13; IFN-alpha-2; IFN-alpha-6; IFN-alphaA; IFN-alphaD; IFN-gamma; IFNα; INFA2; interferon; interferon alpha; interferon alpha 1; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha 1b; interferon alpha 2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); interferon alpha protein; interferon alpha-1; Interferon alpha1 (IFNA1); interferon alpha-1 precursor; interferon alpha-1/13; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-2; Interferon alpha-A; Interferon alpha-D; Interferon alpha-D (IFN alphaD); interferon type A1/A2; interferon type A3; interferon, alpha 1; interferon, alpha 2; interferon-alpha; interferon-alpha 1; interferon-alpha1; interferon-alpha-6; leIF A; LeIF D; MGC138207; MGC138505; MGC138507; MuIFN-alpha-A; novel interferon alpha (Ifna) family member; OTTMUSG00000007655; IFL; IFN; IFN-ALPHA; IFN-alphaD3; IFNA@; IFNA1;IFN-alphaA; IFNAB; INFA2; IFNA2
Immunassay-Kit-Format Matched Antibody Pair
Produkttyp ELISA
Menge 10 x 96 Tests
Probentyp Plasma, Serum, Supernatant
Regulatorischer Status RUO
Primär oder sekundär Primary
Zielspezies Human
Zur Verwendung mit (Geräte) Absorbance Microplate Reader
Inhalt und Lagerung 1 vial (1.1 mL) monoclonal Coating Antibody to human IFN-alpha (100 ug/mL)
1 vial (55 uL) HRP-Conjugate anti-human IFN-alpha monoclonal antibody
1 vial (50 uL) human IFN-alpha Standard protein, 50 ng/mL
Gen-ID (Entrez) 3439, 3440
Gensymbol IFNA1, IFNA2
Kitinhalt Capture (coating) Antibody, Detection Antibody, Standard
Marker oder Farbstoff HRP
Protein-Aliase IFN-alpha-1/13, Interferon alpha-D, LeIF D,IFN-alpha-2, Interferon alpha-A, LeIF A
Kennzeichnung RUO
Lagerungsbedingungen -20°C
Ziel IFN alpha
Prüfzeit 1 hr 10 min
Gesamte Assay-Zeit 24 hr
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