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  7. Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

Codice prodotto. 10229194 Sfoglia Tutto Applied Biosystems Prodotti
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N. di reazioni:
100 Reactions
500 Reactions
Dimensione della confezione:
200 reazioni
Pezzo
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Codice prodotto. N. di reazioni Dimensione della confezione
10229194 500 Reactions 200 reazioni
10734227 100 Reactions Pezzo
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Codice prodotto. 10229194 Fornitore Applied Biosystems™ N. del fornitore 4389986
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966.00 CHF valido fino al 2026-12-18
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Descrizione

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:

  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.

Order Info

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY

Specifica

Content And Storage Sufficient for 500 reactions at 20 μL or 200 reactions at 50 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Metodo di rilevamento SYBR
PCR Method 1-step RT-qPCR
Polimerasi AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Da utilizzare con (apparecchiatura) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Label or Dye SYBR Green
Passive Reference Dye ROX (Pre-mixed)
Linea di prodotti RNA-to-CT, SYBR
Product Type One-Step qRT-PCR Kit
Purity or Quality Grade UP (Ultra Pure)
Quantità 500 Reactions
Reverse Transcriptase ArrayScript™ UP
Run Time Standard
Shipping Condition Wet Ice
Sufficiente per 500 Reactions at 20 μL
Da utilizzare con (applicazione) Gene Expression
N. di reazioni 500 Reactions
Tipo di campione RNA
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For Research Use Only. Not for use in diagnostic procedures.

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