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Invitrogen™ Human SCF Recombinant Protein
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Menge:
10 μg
Packungsgröße:
10 Mikrogramm
Beschreibung
For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Reconstitute lyophilized material in 0.1 mL of 10 mM Acetic acid to a final concentration of 0.1 mg/mL. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C. Avoid multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
Spezifikation
Spezifikation
| Zugriffsnummer | P21583 |
| Zur Verwendung mit (Anwendung) | Bioactivity |
| Zusammensetzung | 0.01M acetic acid with no preservative |
| Gen-ID (Entrez) | 4254 |
| Molekulargewicht | 18.4 kDa |
| Name | Human SCF |
| Reinigungsverfahren | Purified |
| Menge | 10 μg |
| Quelle | E. Coli |
| Lagerungsbedingungen | -20°C, Avoid Freeze/Thaw Cycles |
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