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ACROBiosystems Human PH20 / SPAM1 Protein, His Tag (active enzyme)
Marke: ACROBiosystems PH0-H5225-25ug
Additional Details : Gewicht : 0.00001kg
Beschreibung
Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.Spezifikation
NP_694859.1 | |
25 μg | |
Leu 36 - Tyr 482 | |
SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15 | |
Unconjugated | |
His Tag | |
Less than 1.0 EU / μg by the LAL method. | |
Powder | |
>95% as determined by SDS-PAGE |
53.0 kDa | |
Human PH20, His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1). | |
-20°C | |
PH20 | |
Human | |
50 mM Tris, 100 mM NaCl, pH7.5 | |
HEK293 | |
Enzymes |