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Invitrogen™ Human Mylein Basic Protein ELISA Kit

Artikelnummer. 30826228
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96 Tests
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30826228 96 Tests
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Artikelnummer. 30826228 Lieferant Invitrogen™ Lieferanten-Nr. EEL167
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    595.58 CHF / 96 Stück
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Verfügbar ab: 28-08-2026
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Beschreibung

ELISA

The Human Myelin Basic Protein (MBP) ELISA quantitates MBP in serum, plasma and other biological fluids. Principle of the method The Human Myelin Basic Protein (MBP) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
TRUSTED_SUSTAINABILITY

Spezifikation

Zugriffsnummer P02686
Assay-Bereich 15.63-1000 pg/mL
Assay-Empfindlichkeit: 9.38 pg/mL
Konjugat HRP
Immunassay-Kit-Format Sandwich ELISA Kit
Produkttyp ELISA
Probentyp Cell Lysates, Plasma, Serum, Tissue Homogenates
Zielspezies Human
Zur Verwendung mit (Geräte) Absorbance Microplate Reader
Gen-Alias 20 kDa microtubule-stabilizing protein; C76307; golli mbp; Golli-mbp; Golli-MBP; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; Hmbpr; MBP; MBP S; Mbps; MGC99675; microtubule-stabilizing protein; mld; MOBP; Myelin A1 protein; myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; myelin deficient; myelin membrane encephalitogenic protein; Myelin P1 protein; myelin-associated oligodendrocyte basic protein; R75289; shi; shiverer; Unknown (protein for IMAGE:7984318); unnamed protein product20 kDa microtubule-stabilizing protein; C76307; golli mbp; Golli-mbp; Golli-MBP; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; Hmbpr; MBP; MBP S; Mbps; MGC99675; microtubule-stabilizing protein; mld; MOBP; Myelin A1 protein; myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; myelin deficient; myelin membrane encephalitogenic protein; Myelin P1 protein; myelin-associated oligodendrocyte basic protein; R75289; shi; shiverer; Unknown (protein for IMAGE:7984318); unnamed protein product;
Gen-ID (Entrez) 4155
Gensymbol MBP
Interassay-VK <10%
Intraassay-VK <10%
Kitinhalt Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Marker oder Farbstoff Biotin
Menge 96 Tests
Kennzeichnung RUO
Probenvolumen Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
Versandbedingung Wet or Dry Ice
Lagerungsbedingungen Refer to product documentation for component specific storage temperature.
Ziel MBP
Prüfzeit 1 hr 20 min
Gesamte Assay-Zeit 3 hr 30 min
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