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Invitrogen™ Human MMP13 ELISA Kit
Beschreibung
The Human Matrix MetalloProteinase 13 (Hu MMP13) ELISA quantitates Hu MMP13 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP13. Principle of the method The Human MMP13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Spezifikation
Spezifikation
| Zugriffsnummer | Gehäuse P45452 |
| Assay-Bereich | 8.23 bis 6000 pg/ml |
| Assay-Empfindlichkeit: | 6 pg/mL |
| Kalibrierbereich | 6 bis 6000 pg/ml |
| Assay | Festphasen-Sandwich-ELISA |
| Konjugat | Biotin |
| Produkttyp | ELISA-Kit |
| Proteinfamilie | Zellstruktur- und Organellen-Proteine |
| Probentyp | Plasma, Serum, Überstand |
| Spezifität | Dieses ELISA-Kit zeigt keine Kreuzreaktivität mit einem der getesteten Zytokine: humanes Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3. |
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