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HIF-1 alpha Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody
Marke: Novus Biologicals NB100-449
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Beschreibung
HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Knockout Validated, Knockdown Validated.
Spezifikation
| HIF-1 alpha | |
| Polyclonal | |
| Unconjugated | |
| TBS, 0.1% BSA with 0.09% Sodium Azide | |
| AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp | |
| Rabbit | |
| 93 kDa | |
| 0.1 mL | |
| Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators | |
| 3091 | |
| Human, Mouse, Rat, Canine, Chicken, Goat, Primate, Monkey | |
| IgG |
| Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown | |
| 0.2 mg/mL | |
| Western Blot 1:2000 - 1:10000, Simple Western 1:200, Flow Cytometry 0.125 ug per 1 million cells in a 150 mcl reaction, ELISA 1:100-1:2000, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 2-5 ug/mg lysate, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated, Knockdown Validated | |
| Q16665 | |
| HIF1A | |
| The immunogen recognized by this HIF-1 alpha Antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 [Uniprot# Q16665]. | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Monkey (COS-7) and Rat (review feedback). Has been shown to detect HIF-1 alpha in both mouse and human tissue by IHC with Citrate buffer antigen retrieval. Based on 100% sequence identity, this antibody is expected to react with: Cavia porcellus, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Grass carp, Northern pike, Atlantic cod, Duckbill platypus and Gansu zokor. | |
| Store at 4C. Do not freeze. |
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