HELIOS Monoclonal Antibody (22F6), eFluor™ 450, eBioscience™, Invitrogen™

Beschreibung

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a domit-negative function that has also been found to be upregulated in lymphoid maligcies, including T-acute lymphoblastic leukemia. 22F6 has been reported to cross react with rat Helios. Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using Foxp3 Fixation and Permeabilization Buffers (cat. 00-5521). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 μL (0.03 μg) per test.