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Descrizione
- Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins
- This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies
- This can be used as the first step in refolding proteins or enzymes into their active form
- Urea and dithiothreitol (DTT) may also be necessary
- Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties
- It is an inhibitor of RNase
- Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins
- It apparently disrupts hydrogen bonds which hold the protein in its unique structure; however, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions
Specifica
Spezifikation
| Materiale o nome chimico | GUANIDINE HYDROCHLORIDE |
| Punto di fusione | 180°C to 190°C |
| Colore | White |
| Formula lineare | CH5N3·HCl |
| Quantità | 1 kg |
| Informazioni di solubilità | Soluble in water (6 M - clear, colorless solution). |
| Peso formulazione | 95.53 |
| Percent Purity | ≥99% |
| Grado | Ultra Pure |
| Forma fisica | Crystalline Powder |
Name des Produkts
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