Invitrogen™ Human GM-CSF, Recombinant Protein

Beschreibung

Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Recombinant Protein, RGMCSF20, from Invitrogen.

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). This product cross-reacts with bacteria, Mouse, Leech, Porcine, and Chicken GM-CSF. For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Sterile-filtered through a 0.2 micron filter. Activity: One unit of activity is < 0.1ng/mL, as determined in a proliferation assay of human TF-1 cells, corresponding to a specific activity of > 1x10^7 units/mg. Reconstitute with 0.5 mL of distilled water.