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Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555, Invitrogen™

Goat Polyclonal Secondary Antibody

Marke:  Invitrogen A32727

450.30 CHF gültig bis 2024-03-29
Benutzen Sie den Promotions-Code "21653" um Ihren Promotions-Preis zu erhalten.



Additional Details : Gewicht : 0.00001kg

Artikelnummer. 15698545

  • 474.00 CHF / 1 Milligramm
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Beschreibung

Beschreibung

To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Mouse IgG (H+L) Highly Cross-Adsorbed Polyclonal antibody specifically detects Mouse IgG (H+L) Highly Cross-Adsorbed in Mouse samples. It is validated for Immunocytochemistry, Western Blot.
Spezifikation

Spezifikation

Mouse IgG (H+L) Highly Cross-Adsorbed
Polyclonal
Alexa Fluor Plus 555
Goat
Affinity chromatography
RUO
Mouse
Liquid
Immunocytochemistry, Western Blot
2 mg/mL
proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
Gamma Immunoglobins Heavy and Light chains
1 mg
Secondary
4° C, store in dark
IgG
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote