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AcroBiosystems GMP Human IL-18 Protein
Beschreibung
Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.
Spezifikation
Spezifikation
| Zugriffsnummer | Uniprot |
| Charakterisierung | IL-18 Structure This protein carries no tag. The protein has a calculated MW of 18.2 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE). |
| Format | Powder-GMP |
| Zusammensetzung | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation. |
| Qualitätskontrollen | <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative |
| Menge | 10 mg |
| Quelle | GMP Human IL-18 Protein (GMP-L18H16) is expressed from E. coli cells. It contains AA Tyr 37 - Asp 193 (Accession # ). Request for sequence |
| Biologische Aktivität | GMP Human IL-18 Protein (Cat. No. GMP-L18H16) stimulates secretion of IFN-γ by KG-1 cells. The specific activity of GMP Human IL-18 Protein is >3.00x10^6 IU/mg, which is calibrated against WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) (QC tested).∣The activity of GMP Human IL-18 Protein (Cat. No. GMP-L18H16) was higher than other competing products.∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-18 Protein can promote the expansion of these cells with a reasonable cell viability. Notably, the cells exhibit better expansion in GMP Human IL-18 Protein (ACROBiosystems).∣Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), GMP Human IL-18 Protein (ACROBiosystems, Cat. No. GMP-L18H16) and GMP Human 4-1BB Ligand Protein (ACROBiosystems, Cat. No. GMP-41LH26), in CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3103) for two weeks. The result shows that GMP Human IL-18 Protein (ACROBiosystems) can be comparable with the Competitor M IL-18 protein.∣The Cell based assay shows that GMP Human IL-18 Protein (Cat. No. GMP-L18H16) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-18.∣MALDI-TOF analysis of GMP Human IL-18 Protein (Cat. No. GMP-L18H16), showing a major peak at 18217.2 Da. The minor peak following the major peak is a matrix-associated artifact of the MALDI-TOF (Routinely tested). |
| Spezies | Human |
| Sterilität | Sterile |
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