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Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Marke:  Invitrogen™ 14-5902-81

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Artikelnummer. 15277587

  • 108.00 CHF / 50 Mikrogramm
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Verfügbar ab: 28-08-2025
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Beschreibung

Beschreibung

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
TRUSTED_SUSTAINABILITY
Spezifikation

Spezifikation

GL7
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Rat
50 μg
Primary
4°C
Liquid
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
GL-7 (GL7)
Unconjugated
GL7 antigen; Ly77; Ly-77
Affinity chromatography
RUO
Human, Mouse
Antibody
IgM
Produktvorschläge

Produktvorschläge

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Dokumentation

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Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™ > 50 μg; Unconjugated

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