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Descrizione
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, TrypLE Express is:
- Gentle on cells
- Room temperature-stable
- Easy to use
- Animal origin-free (AOF)
This TrypLE Express is modified as follows:
With: EDTA
Without: Phenol Red
The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room temperature-stable
TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Order Info
Shipping Condition: Room Temperature
Compliance
Manufactured at a cGMP-compliant facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifica
Specifica
| Chelators | EDTA |
| Content And Storage | Storage conditions: 15°C to 30°C, protect from light Alternate storage conditions: 2°C to 8°C or -5°C to -20°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
| Tipo di cellula | Mammalian Cells |
| Classificazione | Animal Origin-free |
| Concentrazione | 1 X |
| Culture Type | Adherent Cell Culture |
| Forma | Liquid |
| Product Type | Cell Culture Dissociation Reagent |
| Sterilità | Sterile-filtered |
| Senza additivi | No Phenol Red |
| Vedi altri risultati |
For Research Use Only. Not for use in diagnostic procedures.
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