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Gibco™ RPMI 1640 Medium (ATCC modification)

Codice prodotto. 11504566 Sfoglia Tutto Gibco Prodotti
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Quantità:
1 x 500 mL
Dimensione della confezione:
500mL
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Codice prodotto. Quantità Dimensione della confezione
11504566 1 x 500 mL 500mL
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Codice prodotto. 11504566 Fornitore Gibco™ N. del fornitore A1049101
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Requires

Supplementation

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With: L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, High glucose, Low sodium bicarbonate

The complete formulation is available.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has been modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, and low L-glutamine. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (1.5 g/L) and therefore requires a 5–7% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

TRUSTED_SUSTAINABILITY

Specifica

Linea cellulare HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Tipo di cellula Mammalian Cells
Classificazione Animal Origin-free
Concentrazione 1X
Forma Liquid
Product Type RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level Standard Serum Supplementation
Sterilità Sterile-filtered
Sterilization Method Sterile-filtered
Con additivi High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate, Low Sodium Bicarbonate
Senza additivi No HEPES, No Sodium Pyruvate
Da utilizzare con (applicazione) Mammalian Cell Culture
Qualità di produzione cGMP-compliant under the ISO 13485 standard
Linea di prodotti Gibco
Quantità 1 x 500 mL
Shipping Condition Ambient
Formato Bottle
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Environment CO2
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For Research Use Only. Not for use in diagnostic procedures.

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