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Gibco™ DMEM, high glucose, pyruvate

Codice prodotto. 13345364 Sfoglia Tutto Gibco Prodotti
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Codice prodotto. Quantità Dimensione della confezione
13345364 1 x 500 mL 500mL
13476146 10 x 500 mL 5L
17825170 1 x 1000 mL 1000mL
11350882 1 x 5 L Pezzo
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Codice prodotto. 13345364 Fornitore Gibco™ N. del fornitore 11995065
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Descrizione

Requires

Supplementation

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate

Without: HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

TRUSTED_SUSTAINABILITY

Specifica

Linea cellulare HeLa, 293, Cos-7, and PC-12
Tipo di cellula Mammalian Cells
Classificazione Animal Origin-free
Concentrazione 1X
Forma Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Standard Serum Supplementation
Sterilità Sterile-filtered
Sterilization Method Sterile-filtered
Con additivi High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Senza additivi No HEPES
Da utilizzare con (applicazione) Mammalian Cell Culture
Glucose Concentration 4500 mg/L
Qualità di produzione cGMP-compliant under the ISO 13485 standard
Linea di prodotti Gibco
Quantità 1 x 500 mL
Shipping Condition Ambient
Formato Bottle
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Environment CO2
Culture Type Mammalian Cell Culture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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