Thermo Scientific™ GeneBLAzer™ LXR Alpha DA Assay-Kit

GeneBLAzer™ LXR alpha DA (Division Arrested) cells and LXR alpha-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-alpha(LXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase.

Division Arrested (DA) cells are available in two configurations:

• Assay kit—includes cells and sufficient substrate to analyze 1 x 384-well plate
• Tube of cells—sufficient to analyze 10 x 384-well plates

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both LXR alpha DA cells and LXR alpha-UAS-bla HEK 293T 293 cells are functionally validated for Z' and EC₅₀ concentrations of TO901317. In addition, LXR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.