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GARP Rat anti-Mouse, Super Bright 600, Clone: YGIC86, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 63-9891-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15983574

  • 147.00 CHF / 25 Mikrogramm


Verfügbar ab
09-10-2020
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Beschreibung

Beschreibung

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.
Spezifikation

Spezifikation

GARP
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
YGIC86
Super Bright 600
Lrrc32
Lrrc32
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
434215
Videos
Dokumentation