Melford G-418 Disulfate

G-418 is derived from the bacterium Micromonospora rhodorangea and belongs to the class of aminoglycoside antibiotics. It acts by inhibiting protein synthesis in cells, specifically targeting the 80S ribosome and interfering with translocation.

In the life science marketplace, G-418 Disulfate finds extensive use as a selective agent for cells that have been genetically modified or transfected with a G-418 resistance gene. This resistance gene is often incorporated into plasmid vectors or recombinant DNA constructs to allow for the selection and maintenance of cells that have successfully taken up the desired genetic material.

The selective nature of G-418 Disulfate makes it an invaluable tool in molecular biology research. By incorporating the G-418 resistance gene and applying G-418 treatment, researchers can specifically select cells that have successfully integrated the desired genetic material.

This antibiotic selection system is commonly used in techniques such as stable cell line generation, gene expression studies, and protein production.