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Foxp3 Rat, PE, Clone: PCH101, Staining Set, eBioscience™
Antibody Staining Kit
Marke: Invitrogen 72-5776-40
Includes:
- Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.
- Fixation/Permeabilization Diluent (cat. 00-5223): 100mL, store at 2-8°C.
- Permeabilization Buffer (10X) (cat. 00-8333): 100mL, store at 2-8°C. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
- Normal Rat Serum (cat. 24-5555): 100 μL, store at 2-8°C.
- Anti-Human Foxp3 PE (clone PCH101, cat. 12-4776): 25 tests, store at 2-8°C. Light-sensitive material.
Beschreibung
This Anti-Human Foxp3 Staining Set contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells. The PCH101 monoclonal antibody reacts with the amino terminus of human Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.The PCH101 antibody crossreacts with rhesus, chimpanzee, and cynomolgus Foxp3. PCH101 recognizes a different epitope of Foxp3 than clones 236A/E7 and 150D/E4.
Spezifikation
| Foxp3 | |
| Monoclonal | |
| PE | |
| Rat | |
| RUO | |
| Chimpanzee, Cynomolgus, Human, Macaque, Monkey, Non-human Primate, Rhesus Monkey | |
| IgG2a κ |
| Flow Cytometry | |
| PCH101 | |
| Forkhead Box P3, Scurfin, JM2 | |
| Kit | |
| Primary | |
| Store at 2-8°C. Do not freeze. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle. |
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