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Description
Thermo Scientific™ dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM. All nucleotides have greater than 99% purity and are free of nuclease activities and human and E. coli DNA. The mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. dNTP/dUTP Mix is designed for direct use in PCR with Taq DNA Polymerase, reverse transcription, and a wide variety of molecular applications.
Features
- Greater than 99% purity confirmed by HPLC
- Free of human and E. coli DNA
High stability
- Stable for a minimum 100 freeze-thaw cycles
- Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
- Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)
Applications
- cDNA synthesis and RT-PCR
- PCR, qPCR
- Standard PCR
- DNA Sequencing
Note: dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.
Spécification
Spécification
| Concentration | 2 mM |
| Contenu et stockage | dNTP/dUTP Mix contains dATP, dCTP, and dGTP at a final concentration of 2 mM and dUTP at 4 mM (1 mL) Store at –20°C. |
| Forme | Liquide |
| Polymérase | ADN polymérase |
| Marqueur ou colorant | Non marqué |
| Pureté ou qualité | >99% |
| Quantité | 1 mL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | PCR standard, RT-PCR, Real Time PCR (qPCR) |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
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