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F4/80 Monoclonal Antibody (BM8), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 14-4801-81

94.00 CHF gültig bis 2024-03-29
Benutzen Sie den Promotions-Code "21615" um Ihren Promotions-Preis zu erhalten.



Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15277387

  • 141.00 CHF / 50 Mikrogramm
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Beschreibung

Beschreibung

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80. Applications Reported: The BM8 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of paraffin embedded tissue sections. Immunoblotting must be performed under non-reducing conditions. For immunohistochemical staining of paraffin sections, proteinase treatment is needed. It has been reported that reduction with 2-mercaptoenthanol destroys the BM8 antigen. Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse spleen or bone marrow cells.

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Spezifikation

Spezifikation

F4/80
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q61549
ADGRE1
Affinity chromatography
RUO
13733
4° C
Liquid
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
BM8
Unconjugated
ADGRE1
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
Rat
50 μg
Primary
Mouse
Antibody
IgG2a κ
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote

For Research Use Only.