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Invitrogen™ F(ab')2-Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, PE

Artikelnummer. 10312284
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Artikelnummer. 10312284 Lieferant Invitrogen™ Lieferanten-Nr. 31860

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F(ab')2-Donkey Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31860 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31860 reacts with the heavy chains of goat IgG and with light chains common to most goat immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins. However, these antibodies may cross-react with immunoglobulins from other species. Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 82.0 at 566 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) Phycoerythrin Source: Seaweed Store product protected from light at 4°C until opened. DO NOT FREEZE. RPE Amax= 490 nm, 545 nm, and 566 nm; Emax= 580 nm. Reconstitute with 1.0 mL of distilled water (0.5 mg/mL after restoration). Country of Origin: USA.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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Spezifikation

Antigen Goat IgG (H+L) Cross-Adsorbed
Anwendungen Flow Cytometry
Klassifikation Polyclonal
Konzentration 0.5 mg/mL
Konjugat PE
Zusammensetzung PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
Wirtsspezies Donkey
Reinigungsverfahren Antigen affinity chromatography
Menge 1 mL
Regulatorischer Status RUO
Primär oder sekundär Secondary
Zielspezies Goat
Inhalt und Lagerung 4°C, do not freeze
Produkttyp Secondary Antibody
Form Lyophilized
Isotype IgG
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