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Gibco™ Expi293™ GnTI- Expression System Kit
Descrizione
System kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.
Features:
- Homogeneous N-glycosylation of expressed proteins
- Equivalent protein yields to parental Expi293F cells - up to 1 g/L
- Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
- Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
- High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
Components included:
- 1 vial of frozen Expi293F GnTI- cells
- 1 L of Expi293 Expression Medium
- 1 ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture
- 100 mL Opti-Plex Complexation Buffer
- 1 PNGase F Glycan Cleavage kit
- pRABBIT IgG IRES-EmGFP positive control vector
All components of the Expi293 GnTI- Expression System are also available for individual purchase
Specifica
Specifica
| Tipo di erogazione | Transfection |
| Quantità | 1 kit |
| Content And Storage | • Expi293F GnTI- Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen • 1 L Expi293 Expression Medium, store at 2–8°C (protect from light) • ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C • 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light) • PNGase F Glycan Cleavage Kit, store at -20°C • pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
| Linea cellulare | Expi293F™ |
| Linea di prodotti | Expi293 |
| Da utilizzare con (applicazione) | Protein Production |
For Research Use Only. Not for use in diagnostic procedures.
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