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  6. Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

Product Code. 18094070 Shop All Thermo Scientific Products
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Product Code. 18094070 Supplier Thermo Scientific™ Supplier No. A65393
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Description

The Thermo Scientific™ EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

The Thermo Scientific™ EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

Features of the EquiPhi29 DNA Amplification Kit include:

  • Fast DNA amplification in less than two hours
  • Sensitivity from 1 fg of input DNA
  • Maximal yield of 17 μg amplified DNA
  • Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
  • Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
  • All required components for DNA amplification in RCA or MDA-WGA reactions

The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.

TRUSTED_SUSTAINABILITY

Specifications

Produkttyp DNA Amplification Kit
Form Flüssig
Zur Verwendung mit (Anwendung) RCA, MDA-WGA
Polymerase EquiPhi29 DNA Polymerase
Menge 100 Reaktionen
Forschungskategorie Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Inhalt und Lagerung

• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)
• 0.1 M DTT (0.25 mL)
• 500 μM Exo-Resistant Random Primer (0.2 mL)
• 10 mM dNTP Mix (0.2 mL)
• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)

Store at -15 to -25°C.
Format Kit
Optimale Reaktionstemperatur 42 °C
Versandbedingung Trockeneis
Reaktionszeit Up to 2 hours
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Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

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