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EOMES Rat anti-Mouse, PerCP-eFluor™ 710, Clone: Dan11mag, eBioscience™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 46-4875-82

Additional Details : Gewicht : 0.25000kg

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Artikelnummer. 15331330

  • 401.00 CHF / 100 Mikrogramm


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Beschreibung

Beschreibung

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenpcytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP- eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
Spezifikation

Spezifikation

EOMES
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O54839
Eomesodermin homolog; EOMES; TBR2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
Dan11mag
PerCP-eFluor™ 710
Eomes
Liquid
Eomes
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
13813
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.