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ACROBiosystems Ebolavirus (subtype Bundibugyo, strain Uganda 2007) Envelope Glycoprotein 1 (GP1) Protein, His Tag
Marke: ACROBiosystems BEP-V5221-1mg
Additional Details : Gewicht : 0.00010kg
Beschreibung
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE).Spezifikation
31.8 kDa | |
Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1, His Tag (BEP-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Gln 304 (Accession # ACI28624). | |
-20°C | |
Glycoprotein / GP (virus) | |
Ebolavirus | |
PBS, pH7.4 | |
HEK293 | |
Viral Proteins |
1 mg | |
Ile 33 - Gln 304 | |
GP,Envelope glycoprotein,GP(1,2) | |
Unconjugated | |
His Tag | |
Less than 1.0 EU / μg by the LAL method. | |
Powder | |
>90% as determined by SDS-PAGE |