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Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit
Descrizione
Includes
10mL Dynabeads oligo (dT)25, 60mL Lysis/Binding Buffer, 120mL Washing Buffer A, 60mL Washing Buffer B, 15mL 10mM Tris-HCl (Elution buffer), Contains enough reagents for 40 standard isolations.
The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.
Advantages of the Dynabeads mRNA DIRECT Kit:
- Fast – 15 minute procedure yields pure, intact mRNA
- Highly pure mRNA isolation – best choice upstream of cDNA synthesis
- Sensitive mRNA isolation – enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)
System overview
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads. Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads Oligo (dT)25 beads (200 μL) binds up to 2 μg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.
Order Info
Shipping Condition: Room Temperature
Specifica
Specifica
| Content And Storage | • 10 mL Dynabeads Oligo (dt)25, 4°C |
| Isolation Technology | Magnetic Bead |
| Volume di eluizione | 10 to 100 μL |
| Tipo di campione | Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood |
| Tipo di prodotto finale | mRNA |
| Compatibilità ad alta produttività | High-throughput Compatible |
| Da utilizzare con (apparecchiatura) | Automated Liquid Handling Systems |
| Da utilizzare con (applicazione) | RT-PCR, qPCR, cDNA library construction, microarray analysis |
| Quantità | 10 mL |
| Shipping Condition | Room Temperature |
| Vedi altri risultati |
Sicurezza e movimentazione
- Dynabeads mRNA DIRECT Purification Kit
- Attenzione
- Lesione oculare grave/irritazione oculare Categoria 2
- H319-Provoca grave irritazione oculare.
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
- MIXTURE LIST-Contain: Lithium chloride
For Research Use Only. Not for use in diagnostic procedures.
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