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  6. Thermo Scientific™ Dynabeads™ CaptureSelect™ AAVX Magnetic Beads

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAVX Magnetic Beads

Produktkod. 30276387 Handla allt Thermo Scientific Produkter
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Kapazität (metrisch):
1 ml
5 ml
50 ml
Förpackningsstorlek:
1 Milliliter
1 Stück
50 Milliliter
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30276684 5 ml 1 Stück
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Produktkod. 30276387 Leverantör Thermo Scientific™ Leverantörsnummer 2853522001

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Beskrivning

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAVX Magnetic Beads are high-capacity, high-throughput magnetic affinity particles for the purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications.

Dynabeads CaptureSelect AAVX Magnetic Beads are high-capacity, high-throughput magnetic affinity particles for the purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. The anti-AAVX affinity ligand demonstrates good binding reactivity towards a large variety of AAV serotypes, including AAV1 to AAV8, AAVrh10, and synthetic serotypes. The magnetic beads enable purification using manual and robotic magnetic separators of AAV serotypes directly from crude feedstock in a single step with high purity and yield.

Features of DynaBeads CaptureSelect AAVX Magnetic Beads include:

  • One-magnetic-step AAV purification from crude material with high purity and yield
  • Compatible with manual and automated workflows
  • High specificity and capacity
  • Easy upscaling to higher volumes
  • Non-animal derived

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a ∼12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Bead matrix: superparamagnetic
  • Bead size: 4.5 μm
  • Ligand: CaptureSelect AAVX affinity ligand
  • Binding capacity: ∼1 x1012 viral particles/mg beads
  • Elution conditions: 50 mM citric acid pH 2.5-3.0
  • Formulation buffer: tris-buffered saline, 0.05% Tween 20, 0.05 % sodium azide

Specifikationer

Bindungsvermögen ∼1 x 1012 viral particles/mg beads
Kapazität (metrisch) 1 ml
Konzentration 30 mg beads/mL slurry, 1 mg contains approximately 1.3 x 107 beads
Zusammensetzung Tris-buffered saline with 0.05% Tween-20 and 0.05% Sodium Azide
Matrix Non-porous monodisperse superparamagnetic beads
Inhalt und Lagerung Store at 2–8°C.
Partikelgröße 4.5 μm
Form Resin Slurry
Produktlinie CaptureSelect
Menge 1 mL
Zur Verwendung mit (Anwendung) Magnetic isolation of AAV capsids
Ziel Multiple AAV serotypes
Ligandentyp AAVX Affinity Ligand
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