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  6. Thermo Scientific™ Dynabeads™ CaptureSelect™ AAV9 Magnetic Beads

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAV9 Magnetic Beads

Product Code. 30275957 Shop All Thermo Scientific Products
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50 Milliliter
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Product Code. 30275957 Supplier Thermo Scientific™ Supplier No. 2853332001

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Description

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAV9 Magnetic Beads are high-capacity, high-throughput magnetic affinity particles for the purification of naturally occurring and synthetic adeno-associated virus serotype 9 (AAV9) used for gene therapy applications.

Dynabeads CaptureSelect AAV9 Magnetic Beads are high-capacity, high-throughput magnetic affinity particles for the purification of naturally occurring and synthetic adeno-associated virus serotype 9 (AAV9) used for gene therapy applications. The anti-AAV9 affinity ligand demonstrates good binding reactivity towards the AAV9 serotype. The magnetic beads enable purification using manual and robotic magnetic separators of the AAV9 serotype directly from crude feedstock in a single step with high purity and yield.

Features of DynaBeads CaptureSelect AAV9 Magnetic Beads include:

  • One-magnetic-step AAV purification from crude material with high purity and yield
  • Compatible with manual and automated workflows
  • High specificity and capacity
  • Easy upscaling to higher volumes
  • Non-animal derived

The magnetic beads bind with high affinity specifically to the AAV9 serotype. For the isolation of other AAV serotype capsids we recommend DynaBeads CaptureSelect AAVX magnetic beads (Cat. No. 2853522001, 2853522005, 2853522050).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a ∼12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain. The ligand is efficiently produced in yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Bead matrix: non-porous monodisperse superparamagnetic beads
  • Bead size: 4.5 μm
  • Ligand: CaptureSelect AAV9 affinity ligand
  • Binding capacity: >5 x 1011 viral particles/mg beads
  • Elution conditions: 50 mM citric acid, pH 2.5–3.0
  • Formulation buffer: tris-buffered saline, 0.05% Tween 20, 0.05 % sodium azide

Specifications

Bindungsvermögen >5 x 1011 vp/mg beads
Kapazität (metrisch) 1 ml
Konzentration 30 mg beads/mL slurry, 1 mg contains approximately 1.3 x 107 beads
Zusammensetzung 4 to 6% (v/v) Slurry
Matrix Non-porous monodisperse superparamagnetic beads
Partikelgröße 4.5 μm
Produktlinie CaptureSelect
Menge 1 mL
Zur Verwendung mit (Anwendung) Magnetic isolation of AAV9 capsids
Ziel AAV9 (Adeno-associated serotype 9)
Ligandentyp CaptureSelect™ AAV9 Affinity Ligand
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